Document Details

Document Type : Thesis 
Document Title :
Characterization of a ‎Novel Protein – 3D10 ‎‎– a Secreted Receptor Form of the Human ‎Osteoclast-‎Associated Receptor (OSCAR)
Characterization of a ‎Novel Protein – 3D10 ‎‎– a Secreted Receptor Form of the Human ‎Osteoclast-‎Associated Receptor (OSCAR)
 
Document Language : English 
Abstract : Human osteoclast-associated receptor (hOSCAR) is a member of the leukocyte receptor ‎cluster (LRC) of an unknown ligand. hOSCAR has been reported to be expressed in several ‎mononuclear cells (MNC) of myeloid origin and plays a role in modulating innate and ‎adaptive immune response. We identified an alternative spliced isoform of hOSCAR that we ‎named 3D10. Sequence analysis showed that 3D10 is one of a group of hOSCAR with a ‎non-spliced intron resulting in larger transcripts and soluble proteins lacking a trans-‎membrane domain. Comparisons were made in tissue distribution between the two groups ‎using specific PCR primers and rabbit polyclonal anti-hOSCAR and anti-soluble hOSCAR ‎antibodies. Both groups were found to be differentially expressed in peripheral blood ‎leukocytes and in a wide variety of tissues. They were also found to be expressed in all ‎MNC and neutrophils. The membrane-bound isoforms were down-regulated more than the ‎soluble isoforms following stimulation in MNC with the mitogens PWM, Con A and PHA. ‎Studies using THP-1 cells showed that the soluble isoforms are up-regulated by both PMA ‎and LPS, and that they are secreted and may act as decoy receptors. Performing yeast two ‎hybrid screening of macrophage cDNA library identified potential binding partners that ‎might include hOSCAR ligand and cytoplasmic modulators. Screening mouse tissues with ‎anti-soluble hOSCAR Ab suggests the existence of the soluble group of mOSCAR. Finally, ‎although 3D10 has been found to be secreted, over-expressed intra-cellular 3D10 inhibits ‎NF-κB in a TNF-α-independent pathway.‎ 
Supervisor : Advisor: Patrick Flood 
Thesis Type : Doctorate Thesis 
Publishing Year : 2007 AH
2007 AD 
Number Of Pages : 114 
Added Date : Sunday, February 14, 2010 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عماد خانKhan, Emad InvestigatorDoctorate 

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