|
Document Type |
: |
Article In Journal |
|
Document Title |
: |
Detection of human herpes virus-8 in Kaposi sarcoma and other vascular tumors; Immunohistochemical analysis. Detection of human herpes virus-8 in Kaposi sarcoma and other vascular tumors; Immunohistochemical analysis. |
|
Document Language |
: |
English |
|
Abstract |
: |
BACKGROUND: The diagnostic distinction of some vascular
tumors and Kaposi’s sarcoma (KS) can be sometime difficult.
Recently, a monoclonal antibody to human herpes virus- 8 latent
nuclear antigen-1 has been demonstrated in Kaposi’s sarcoma.
In this study the usefulness of this antibody is evaluated to
differentiate Kaposi’s sarcoma from the other vascular lesions.
METHODS: Formalin-fixed, paraffin-embedded tissue
sections (4 μm) from 20 cases of Kaposi sarcoma, ten cases
of capillary hemangioma (pyogenic granuloma), five cases of
angiosarcoma, three cases of hemangioendothelioma, three
cases of vascular transformation of lymph nodes were examined
immunohistochemically for HHV-8 using mouse monoclonal
antibody (NCL-HHV8-LNA), Novocastra, United Kingdom. It
is specific for human herpesvirus type 8 latent nuclear antigen-
1 (LNA-1). Nuclear staining in tumor cells was considered a
positive result. RESULT: HHV-8 stain showed strong, diffuse,
nuclear staining for human herpes virus 8 latent nuclear antigen-
1 in all, 20/20 cases of Kaposi sarcoma (100%), whereas all cases
of hemangioma, angiosarcoma, hemangioendothelioma and
vascular transformation of lymph nodes were negative for this
antigen. CONCLUSION: Immunohistochemistry for HHV-8 is
a characteristic immunohistochemical marker of Kaposi sarcoma
and considered as a very useful tool for differentiation between
Kaposi sarcoma and other vascular lesions. |
|
ISSN |
: |
0940-5429 |
|
Journal Name |
: |
The New Egyptian Journal of Medicine |
|
Volume |
: |
39 |
|
Issue Number |
: |
5 |
|
Publishing Year |
: |
2008 AH
2008 AD |
|
Article Type |
: |
Article |
|
Added Date |
: |
Sunday, April 11, 2010 |
|
Researchers
| جودة المغربي | Al-Maghrabi, Jaudah | Investigator | Doctorate | |
|